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Image Search Results
Journal:
Article Title: Characterization of a Streptococcal Endopeptidase with Homology to Human Endothelin-Converting Enzyme
doi: 10.1128/IAI.69.1.58-64.2001
Figure Lengend Snippet: Analysis of rPepO during induction and purification. IPTG-induced VT1395 soluble cell extracts (3.5 × 107 cells) were electrophoresed on a 12% acrylamide gel and stained with Coomassie brilliant blue (lane 1). Silver staining of a 10% acrylamide gel containing 250 ng of eluted protein (lane 2) and immunoblot analysis of a 10% acrylamide gel containing 250 ng of eluted protein using rPepO antiserum (100 ng/ml) as a probe for rPepO (lane 3) are also shown.
Article Snippet: Fractions eluted during the purification process were analyzed by
Techniques: Purification, Acrylamide Gel Assay, Staining, Silver Staining, Western Blot